The researchers at the Cambridge Institute for Medical Research and the Medical Research Council Laboratory of Molecular Biology (LMB) in Cambridge studied tiny protein-producing factories, called ribosomes, isolated from cells. They capitalised on improvements made at the LMB to a high-powered imaging technique known as single particle cryo-electron microscopy.
The microscopes, capable of achieving detail near to the atomic level, enabled the team to link the molecular origins of a rare inherited leukaemia predisposition disorder, ‘Shwachman-Diamond Syndrome’ (SDS), and a more common form of acute leukaemia to a common pathway involved in the construction of ribosomes.
The research, which was funded by the blood cancer charity Bloodwise and the Medical Research Council (MRC), is published online in the journal Nature Structural and Molecular Biology.
Ribosomes are the molecular machinery in cells that produce proteins by ‘translating’ the instructions contained in DNA via an intermediary messenger molecule. Errors in this process are known to play a part in the development of some bone marrow disorders and leukaemias. Until now scientists have been unable to study ribosomes at a high enough resolution to understand exactly what goes wrong.
Ribosomes are constructed in a series of discrete steps, like an assembly line. One of the final assembly steps involves the release of a key building block that allows the ribosome to become fully functional. The research team showed that a corrupted mechanism underlying this fundamental late step prevents proper assembly of the ribosome.
This provides an explanation for how cellular processes go awry in both Shwachman-Diamond syndrome and one in 10 cases of T-cell acute lymphoblastic leukaemia. This form of leukaemia, which affects around 60 children and young teenagers a year in the UK, is harder to treat than the more common B-cell form.
The findings from the Cambridge scientists, who worked in collaboration with scientists at the University of Rennes in France, open up the possibility that a single drug designed to target this molecular fault could be developed to treat both diseases.
Professor Alan Warren, from the Cambridge Institute of Medical Research at the University of Cambridge, said: “We are starting to find that many forms of blood cancer can be traced back to defects in the basic housekeeping processes in our cells’ maturation. Pioneering improvements to electron microscopes pave the way for the creation of a detailed map of the how these diseases develop, in a way that was never possible before.”
Single particle cryo-electron microscopy preserves the ribosomes at sub-zero temperatures to allow the collection and amalgamation of multiple images of maturing ribosomes in different orientations to ultimately provide more detail.
The technique has been refined in the MRC Laboratory of Molecular Biology by the development of new ‘direct electron detectors’ to better sense the electrons, yielding images of unprecedented quality. Methods to correct for beam-induced sample movements and new classification methods that can separate out several different structures within a single sample have also been developed.
Dr Matt Kaiser, Head of Research at Bloodwise, said: “New insights into the biology of blood cancers and disorders that originate in the bone marrow have only been made possible by the latest advances in technology. While survival rates for childhood leukaemia have improved dramatically over the years, this particular form of leukaemia is harder to treat and still relies on toxic chemotherapy. These findings will offer hope that new, more targeted, treatments can be developed.”
The research received additional funding from a Federation of European Biochemical Societies (FEBS) Long term Fellowship, the SDS patient charity Ted’s Gang and the Cambridge NIHR Biomedical Research Centre.
For further information, please contact Henry Winter at the Bloodwise Press Office on 020 7269 9019, press mobile 07824 375880, or email: firstname.lastname@example.org
Notes to Editors
The findings are published online in the journal Nature Structural and Molecular Biology on 19 October under the title ‘Mechanism of eIF6 release from the nascent 60S ribosomal subunit’. Corresponding author: Prof Alan J Warren, Cambridge Institute for Medical Research, Cambridge.
Bloodwise is the UK’s biggest blood cancer charity dedicated to improving the lives of patients. The charity, which was formed in 1960, changed its name from Leukaemia & Lymphoma Research in September 2015. Its life-saving work is focused on stopping people from dying of blood cancer, improving the quality of life for patients and their families, and where possible, stopping people getting blood cancer in the first place.
The charity’s research is targeted at understanding more about blood cancer, finding causes, improving diagnosis and treatments, and running groundbreaking clinical trials for patients. The charity champions patients’ needs by influencing relevant decision makers and influencers, and seeking to raise awareness of the issues faced by patients. Their patient services provide information, support and assistance to patients at every stage of their journey.
Around 38,000 people of all ages, from children to adults, are diagnosed with blood cancers and related disorders every year in the UK. It is a complex disease area made up of 137 individual diseases. Some affect thousands of people, such as common forms of leukaemia, lymphoma and myeloma. Others affect only a handful. But together, blood cancers are the fifth most common form of cancer.
For more information visit www.bloodwise.org.uk.
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