New technique could aid treatments for diseases that lead to paralysis
Scientists working to develop new treatments for neurodegenerative diseases have been stymied by the inability to grow human motor neurons in the lab. Motor neurons drive muscle contractions, and their damage underlies devastating diseases such as amyotrophic lateral sclerosis and spinal muscular atrophy, both of which ultimately lead to paralysis and early death.
In new research, scientists at Washington University School of Medicine in St. Louis have converted skin cells from healthy adults directly into motor neurons without going through a stem cell state.
The technique makes it possible to study motor neurons of the human central nervous system in the lab. Unlike commonly studied mouse motor neurons, human motor neurons growing in the lab would be a new tool since researchers can’t take samples of these neurons from living people but can easily take skin samples.
The study is published Sept. 7 in the journal Cell Stem Cell.
Avoiding the stem cell phase eliminates ethical concerns raised when producing what are called pluripotent stem cells, which are similar to embryonic stem cells in their ability to become all adult cell types. And importantly, avoiding a stem cell state allows the resulting motor neurons to retain the age of the original skin cells and, therefore, the age of the patient. Maintaining the chronological age of these cells is vital when studying neurodegenerative diseases that develop in people at different ages and worsen over decades.
“In this study, we only used skin cells from healthy adults ranging in age from early 20s to late 60s,” said senior author Andrew S. Yoo, PhD, an assistant professor of developmental biology. “Our research revealed how small RNA molecules can work with other cell signals called transcription factors to generate specific types of neurons, in this case motor neurons. In the future, we would like to study skin cells from patients with disorders of motor neurons. Our conversion process should model late-onset aspects of the disease using neurons derived from patients with the condition.”
“Going back through a pluripotent stem cell phase is a bit like demolishing a house and building a new one from the ground up,” Yoo said. “What we’re doing is more like renovation. We change the interior but leave the original structure, which retains the characteristics of the aging adult neurons that we want to study.”
The ability of scientists to convert human skin cells into other cell types, such as neurons, has the potential to enhance understanding of disease and lead to finding new ways to heal damaged tissues and organs, a field called regenerative medicine.
To convert skin cells into motor neurons, the researchers exposed the skin cells to molecular signals that are usually present at high levels in the brain. Past work by Yoo and his colleagues — then at Stanford University — showed that exposure to two short snippets of RNA turned human skin cells into neurons. These two microRNAs — called miR-9 and miR-124 — are involved with repackaging the genetic instructions of the cell.
In the new study, the researchers extensively characterized this repackaging process, detailing how skin cells reprogrammed into generic neurons then can be guided into specific types of neurons. They found that genes involved in this process become poised for expression but remain inactive until the correct combination of molecules is provided. After much experimentation with multiple combinations, the researchers found that adding two more signals to the mix — transcription factors called ISL1 and LHX3 — turned the skin cells into spinal cord motor neurons in about 30 days.
The combination of signals — microRNAs miR-9 and miR-124 plus transcription factors ISL1 and LHX3 — tells the cell to fold up the genetic instructions for making skin and unfurl the instructions for making motor neurons, according to Yoo and the study’s co-first authors, Daniel G. Abernathy and Matthew J. McCoy, doctoral students in Yoo’s lab; and Woo Kyung Kim, PhD, a postdoctoral research associate.
Another past study from Yoo’s team showed that exposure to the same two microRNAs, miR-9 and miR-124, plus a different mix of transcription factors could turn skin cells into a different type of neuron. In that case, the skin cells became striatal medium spiny neurons, which are affected in Huntington’s disease — an inherited, eventually fatal genetic disorder that causes involuntary muscle movements and cognitive decline beginning in middle adulthood.
In the new study, the researchers said the converted motor neurons compared favorably to normal mouse motor neurons, in terms of the genes that are turned on and off and how they function. But the scientists can’t be certain these cells are perfect matches for native human motor neurons since it’s difficult to obtain samples of cultured motor neurons from adult individuals. Future work studying neuron samples donated from patients after death is required to determine how precisely these cells mimic native human motor neurons.
This work is supported by the National Institutes of Health (NIH) Director’s Innovator Award, grant number DP2NS083372-01; the Presidential Early Career Award for Scientists and Engineers; the Missouri Spinal Cord Injury/Disease Research Program; the Cure Alzheimer’s Fund; Andrew B. and Virginia C. Craig Faculty Fellowship; a Philip and Sima Needleman Graduate Student Fellowship; a Ruth L. Kirschstein National Research Service Award Institutional Predoctoral Fellowship, grant number T32GM081739; and the Children’s Discovery Institute.
Abernathy DG, Kim WK, McCoy MJ, Lake AM, Ouwenga R, Lee SW, Xing X, Li D, Lee HJ, Heuckeroth RO, Dougherty JD, Wang T, Yoo AS. MicroRNAs induce a permissive chromatin environment that enables neuronal subtype-specific reprogramming of adult human fibroblasts. Cell Stem Cell. Sept. 7, 2017.
Washington University School of Medicine’s 2,100 employed and volunteer faculty physicians also are the medical staff of Barnes-Jewish and St. Louis Children’s hospitals. The School of Medicine is one of the leading medical research, teaching and patient-care institutions in the nation, currently ranked seventh in the nation by U.S. News & World Report. Through its affiliations with Barnes-Jewish and St. Louis Children’s hospitals, the School of Medicine is linked to BJC HealthCare.