Huntington’s disease could alter neurons since they start developing, according to a study conducted by the international HD iPSC Consortium, which counts with the participation of the team of the Laboratory of Stem Cells and Regenerative Medicine of the Department of Biomedicine of the Faculty of Medicine and Health Sciences of the UB, led by Josep M. Canals, from the Faculty of Medicine and Health Sciences of the University of Barcelona and the August Pi i Sunyer Biomedical Research Institute (IDIBAPS). The study, published in the scientific journal Nature Neuroscience, counts with the collaboration of the experts of the UB Mónica Pardo, Gerardo García, Marco Straccia, Phil Sanders and Jordi Alberch.
The study shows that using pluripotent stem cells derived from patients (iPSC) and animal models of the disease- the genes involved in the development of the stretchmark nucleus –the affected structure of the nervous system- are already altered in the process when neurons are created. According to the researchers, these results could change the way to treat the disease –which has no cure yet- since they open doors to new therapies focused on the previous stages of the appearance of symptoms.
Huntington’s disease is a progressive and irreversible neurodegenerative disease caused by the mutation of the gene that codifies the Huntington protein. This is a hereditary rare disease with only five-seven affected people (out of 100.000) in western countries.
According to Josep M. Canals, also researcher at the Institute of Neurosciences of the University of Barcelona and the August Pi i Sunyer Biomedical Research Institute (IDIBAPS), “despite being a genetic disease, so far it was thought that the development of the nervous system of the patients was correct until the patient ages and some symptoms such as motor alterations (chorea, stiffness, etc.), cognitive deficiencies and psychiatric disorders start coming up. Our details show that there are subtle signs of alterations in the development of the neurons of these patients, which are not the same as normal neurons”.
Study with iPSC cells from patients
The first phase of the study, led by the group of the UB, consisted on an analysis of genic expression during the striatal development in animal models, which was later compared to the genic expression of neurons from human iPSC cells. iPSCs are skin cells reprogrammed with transcription factors so that they function as a pluripotent stem cell similar to embryonic cells, with the ability to differentiate in any kind of specialized cell, in this case, neurons. This process enables studying the effects of genetic mutation in the affected cells by the disease.
When researchers crossed genic expression data, they detected that more than 600 genes responsible for the development were altered in Huntington’s patients’ cells. Out of this list of altered genes, they selected the most important ones and then focused on a special pathway, the expression which can be controlled with a drug: ISX-9 molecule. This drug was given in vitro to patient’s cells and also to model mice with neuroprotective effects. “The drug reversed some of the markers: there was an increase of synapse and improvements at a cognitive level in model mice with the disease, which improved in memory tests” says Mónica Pardo, first author of the UB group.
“The results show that neurons in Huntington’s patients, although they have the same function as normal neurons, are not totally the same and by acting on developing altered genes could reverse and lower symptoms” says Josep M. Canals.
New view for the disease
According to the researchers, this study involves a change on the view on Huntington’s disease evolution. “Instead of waiting for the symptoms to come up, maybe we should act on malfunctioning neurons before with drugs or inciting the activity of affected neurons like it is done in other diseases such as Parkinson” says Josep M. Canals.
Among the current objectives of the HD iPSC Consortium is to widen the study with a wider range of patients in order to find out which genes are systematically and uniformly altered among them. Therefore, new precise therapeutic targets can be established in order to create new drugs to treat the disease.
This study is part of the ADVANCE(CAT) project, integrated in the NEXTHEALTH community, coordinated by Biocat and led by the University of Barcelona and Ferrer. ADVANCE(CAT) enables the arrival of advanced therapies from pre-clinical stages towards the industrial production and clinical application and counts with the partial support of ACCIÓ (Catalonia Trade and Investment; Generalitat de Catalunya) and the European Union in the operational program European Regional Development Fund (ERDF) Catalonia 2014-2020.
Universitat de Barcelona