The Confocal Laser Endomicroscope (CLE) contains a powerful microscope which allows clinicians to view the bacteria that are thought to trigger diseases of the bowel such as Crohn’s disease and ulcerative colitis.
“Bacteria within the wall of the gut are already believed to play an important role in the development of inflammatory bowel disease and we now have a powerful new tool for viewing this bacteria during routine colonoscopy,” said Prof Alastair Watson of the University of East Anglia (UEA), who led the work in partnership with researchers in France and Germany.
“This new technique will allow the rapid identification of patients at risk or in the early stages of this common but distressing group of diseases.”
The causes of Crohn’s disease and ulcerative colitis are still not completely understood but bacteria within the mucous membrane of the gut are thought to play a role. The current method of taking biopsies prevents observation of the bacteria’s exact location and of the way it interacts with the mucous membrane. The new endomicroscopy technique, which uses a flourescent dye to highlight the bacteria, allows these exact processes to be viewed at a sub-cellular level during routine colonoscopy.
Funded by the Wellcome Trust, the study also found that of 163 patients studied, those with Crohn’s disease or ulcerative colitis were much more likely to have bacteria within the wall of their gut than those with healthy intestines.
‘Confocal laser endomicroscopy is a new imaging modality for recognition of intramucosal bacteria in inflammatory bowel disease in vivo’ by D Moussata (Claude Bernard University, France), M Goetz (Johannes Gutenberg University, Germany), A Goeckner (Johannes Gutenberg University), M Kerner (Johannes Gutenberg University), B Campbell (University of Liverpool), A Hoffman (Johannes Gutenberg University), S Biesterfeld (Johannes Gutenberg University), B Flourie (Claude Bernard University), J-C Saurin (Claude Bernard University), P Galle (Johannes Gutenberg University), M Neurath (Johannes Gutenberg University), A Watson (University of East Anglia), R Kiesslich (Johannes Gutenberg University) is published online by Gut: http://gut.bmj.com/content/early/2010/10/26/gut.2010.213264.full