Huang’s team created a molecular-level understanding of the process that Lederberg first observed. They used high-resolution microscopes to record time-lapse images of rod-shaped E. coli cells becoming L-form blobs and then reverting to rod-shaped again.
E. coli’s cell wall is knitted together by proteins, a class of molecules that work together to perform many biological functions. “It’s like an orchestra in which several proteins perform different roles required to build the cell wall,” Huang said.
Previous research has shown that one protein, MreB, acts like the conductor, coordinating the efforts of several other proteins. But those studies focused on normal, rod-shaped E. coli. In this experiment, Huang’s team wanted to understand how MreB factored into the process by which L-forms reverted back to normal.
“We had a suspicion that as the conductor of this orchestra during regular growth, MreB might also be critical for reversion,” Huang said.
Under the tireless gaze of their time-lapse microscope, the researchers grew E. coli cells dosed with the antibiotic cefsulodin. A relative of penicillin, cefsulodin prevents E. coli from building cell walls.
The cefsulodin did not kill the E. coli, but as the cells divided and created successive generations, the bacteria lost their rod-shaped walls and became bloblike L-forms.
The bioengineers let the L-forms grow and reproduce for a few hours before flushing out the cefsulodin. All the while they kept these blobs under microscopic surveillance. As the cells continued to reproduce, the time-lapse images showed that later generations slowly regained their rodlike shape.
That experiment documented the process of reversion to standard form. But it did not prove that MreB was essential.
To demonstrate the link between the rod shape and MreB, the engineers performed a variation on this experiment.
After adding cefsulodin and letting the rod-shaped E. coli reproduce to become shapeless L-forms, they once again flushed out the antibiotic.
But this time, they added a different antibiotic that specifically suppressed MreB function. Two hours later, the cell walls returned, as a rigid structure protecting the cell.
I’m always amazed to discover ways in which biology is programmed so robustly.
But this time the cells were still shaped like blobs, and eventually all of these misshapen cells died.
“What we found was very stark: MreB was critical for this reversion process to occur, and without MreB what would happen is that the cells would just expand in size without any notion of their normal shape,” Huang said.
In addition to offering fundamental insights into how cells maintain their structures, Huang said the findings could help researchers understand how some bacteria adapt to stressful environments.
Many antibiotics, including penicillin, target the cell wall. But bacteria can lose their cell walls and then later recover their shapes. This process of reversion might explain how bacteria develop resistance to antibiotics and establish chronic infections. The populations that survive in L-form and revert to their original shape may not be as susceptible to the next dose of antibiotics.
“Better understanding of cell wall construction could lead to better antibiotic strategies,” Huang said. “And I’m always amazed to discover ways in which biology is programmed so robustly.”
Other Stanford authors of the paper are former postdoctoral scholar Tristan Ursell, PhD, and associate research scholar Samantha Desmarais, PhD.
Information about Stanford’s Department of Bioengineering, which also supported the work, is available at http://bioengineering.stanford.edu. The department is jointly operated by the School of Engineering and the School of Medicine.
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