07:52am Saturday 23 September 2017

Research reveals how bacterial predators kill other bugs without killing themselves

The study by the labs of Professor Liz Sockett and Dr Andrew Lovering at the Universities of Nottingham and Birmingham offers insights into early steps in the evolution of bacterial predators and will help to inform new ways to fight antimicrobial resistance. The research is published in Nature Communications.

Bdellovibrio bacteriovoruseats other bacteria, including important pathogens of humans, animals and crops. It attacks them from inside out using enzymes (called DD-endopeptidases) that first loosen the cell walls of prey bacteria and then cause them to round up like a pufferfish, providing space as a temporary home for the predator. However, Bdellovibrio have similar cell walls so why they don’t fall victim of their own attack has been a conundrum, until now.
Click here for full story

The project, funded by the Biotechnology and Biological Sciences Research Council (BBSRC), found that the bacterium uses an ankyrin-type protein called Bd3460 as a shield. It binds to the tip of the enzyme weapons, nullifying their action until they are safely secreted out of the Bdellovibrio and into the prey bacteria.

Dr Andrew Lovering and Ian Cadby at Birmingham University determined the structure of the ankyrin protein using X-ray crystallography and found that it attaches to two DD-endopeptidase weapons to temporarily deactivate them.

“When I first showed this to Liz, she hit the nail on the head by describing it as a decorative “quiff” on top of the endopeptidase” said Dr Lovering. “This covers up the active site of the enzymes that are used to cut cell walls and offers protection to the Bdellovibriountil these weapons are excreted into the prey.”

Carey Lambert, Rob Till and Professor Liz Sockett at The University of Nottingham confirmed the antidote protein’s use when the gene responsible for its production was deleted.

Professor Liz Sockett: “When the bd3460 gene responsible for antidote production was deleted, the Bdellovibrio had no way of protecting itself from its own weapons. When it attacked harmful bacteria with its cell-wall-damaging enzymes it also felt the effects.

“The Bdellovibrio bacteria lacking the bd3460 gene tried to invade the bacteria but suddenly rounded up like pufferfish and couldn’t complete the invasion – the fatter predator cell could not enter the prey cell.”

This is the first paper to discover a ‘self-protection’ protein in predatory bacteria.

Professor Liz Sockett added: “Most bacteria are not predatory and so understanding these mechanisms gives us a glimpse of how predation evolved. In this case it seems that the bd3460 gene was transferred into ancestors of Bdellovibrio, probably when they were beginning to develop as predators.”

Commenting on the potential impact of the study, Dr Andrew Lovering added: “If we are to use Bdellovibrio as a therapeutic in the future, we need to understand the mechanisms underpinning prey killing and be sure that any self-protective genes couldn’t be acquired by pathogens, causing resistance. Brilliantly, Liz and Carey have demonstrated this did not happen with the bd3460 antidote protein, and Ian and I showed how the mechanism works on predator enzymes only – this is a great inter-university collaboration.”

— Ends —

Our academics can now be interviewed for broadcast via our Media Hub, which offers a Globelynx fixed camera and ISDN line facilities at University Park campus. For further information please contact a member of the Communications team on +44 (0)115 951 5798, email mediahub@nottingham.ac.uk or see the Globelynx website for how to register for this service.

For up to the minute media alerts, follow us on Twitter

Notes to editors:  The University of Nottingham has 43,000 students and is ‘the nearest Britain has to a truly global university, with a “distinct” approach to internationalisation, which rests on those full-scale campuses in China and Malaysia, as well as a large presence in its home city.’ (Times Good University Guide 2016). It is also one of the most popular universities in the UK among graduate employers and the winner of ‘Research Project of the Year’ at the Times Higher Education Awards  2014. It is ranked in the world’s top 75 by the QS World University Rankings 2015/16, and 8th in the UK by research power according to the Research Excellence Framework 2014. It has been voted the world’s greenest campus for three years running, according to Greenmetrics Ranking of World Universities.

 
More informationis available from Professor Liz Sockett, School of Life Sciences, The University of Nottingham liz.sockett@nottingham.ac.uk
 
Emma Rayner – Media Relations Manager
Email: emma.rayner@nottingham.ac.uk Phone: +44 (0)115 951 5793 Location: University Park


Share on:
or:

Health news