As a result, there is a greater chance of obtaining a correct diagnosis, also the method is simple and inexpensive. Segerink’s doctoral defence will take place on 4 November 2011.
Concentration and motility
The lab-on-a-chip developed by Segerink measures sperm concentration: the fertility standard states that a millilitre of ejaculate should contain at least 20 million sperm. A second important aspect of fertility is motility. This too can be measured using the lab-on-a-chip. Simple home test kits are already commercially available. These indicate whether the concentration is “above or below the standard value”. These tests are too limited, however, as they do not give accurate concentration readings.
How does it work
On the chip, sperm flow through a liquid-filled channel, beneath electrode “bridges”. When a cell passes beneath one of these electrodes, there is a brief fluctuation in the electrical resistance. These events are counted. To test the reliability of her concentration measurements, Segerink added microspheres (tiny balls) to the liquid. Would the system only count sperm, or would it also register other particles? She found that the method was selective enough to distinguish sperm from microspheres. The system was also able to reliably distinguish white blood cells from other bodies. In addition to being an indicator of sperm quality, the white cell count provides important additional information to gynaecologists.
To swim or not to swim
Finally, sperm movement (motility) is another important measure of quality. A small adjustment of the lab-on-a-chip is all that is needed to sort motile sperm from non-motile sperm, after which both can be counted separately. By measuring sperm motility in this way, the chip offers a truly complete test.
Segerink developed the “fertility chip” in the BIOS Lab-on-a-Chip research group of Prof. Albert van den Berg, in collaboration with the Twente Medical Spectrum. The research group is part of the MESA+ Institute for Nanotechnology of the University of Twente. Various companies (PigGenetics, Blue4Green, R&R Mechatronics, Menzis, and Lionix) also participated in this project, which was funded by the STW Technology Foundation in The Netherlands.
In 2011, Segerink received a Valorisation Grant, as a first step towards establishing a company. This will provide her with a platform for refining the fertility chip and its accompanying read-out device into a market-ready product.