12:33am Wednesday 20 September 2017

Biased segregation of centrosomes in stem cells

Their work, published in Nature Communications, sheds new light on our understanding of the molecular and cellular mechanisms that govern stem cell division and on the understanding of some human diseases such as cancer.

Stem cells are non-differentiated cells that, through a process of asymmetric cell division, produce two completely different cells: a stem cell that is identical to the original one and a differentiated cell with a specific function in the organism. This particular division strategy allows stem cells to self-renew, and also permits the production of large amounts of tissue during development or the regeneration of tissue that has been damaged. When this tightly regulated cell division process gets thrown off balance, tumors can form. Studies like this one may therefore be key to helping researchers understand the molecular basis of some human diseases.

More information on stem cell division

Before cells divide, the centrosomes – components that organise the cell skeleton – must, like DNA, duplicate so that each daughter cell may receive its own set. They replicate in such a way that they produce an older (“mother”) and a younger (“daughter”) set. “Other groups have shown that when centrosomes are removed from stem cells, these cells divide symmetrically and can produce tumours, and these results show that centrosomes play a crucial role in asymmetric division”, explains Januschke, a member of González team and first author of the study.

To look into how centrosomes are distributed during the asymmetric division of stem cells, the scientists used non-differentiated cells, called neuroblasts, from the brains of Drosophila melanogaster larvae. Using photo-conversion of a centrosome protein, a technique that allows monitoring the age of centrosomes during experiments by measuring fluorescence over time, the researchers found that, contrary to what happens in Drosophila male germline stem cells or in stem cells of the developing rodent nervous system, the daughter cell inherits the mother centrosome, while the stem cell gets the daughter centrosome. These findings indicate that inheritance of these organelles is not haphazard but that it follows a set pattern. Exactly why this happens, and what purpose it serves, is still unknown.

In an independent approach, the scientists applied “photo-conversion” to study a protein similar to the human protein Centrobin, a specific marker of daughter centrosomes in vertebrates. Consistent with the previous photo-conversion results, Centrobin-labelled centrosomes were systematically retained by the stem cell upon each division. “We have obtained for the first time a specific marker of daughter centrosomes in Drosophila. This achievement will allow us to further our understanding of this pattern in stem cell division”, says Januschke.

Reference article:
Drosophila neuroblasts retain the daughter centrosome
Jens Januschke, Salud Llamazares, Jose Reina, Cayetano González.
Nature Communications (2011) [doi: 10.1038/ncomms1245]

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