12:36pm Thursday 19 October 2017

New TSRI Metabolomic Platform Reveals Fundamental Flaw in Common Lab Technology

“We found that even relatively low temperatures used in GC-MS can have a detrimental effect on small molecule analysis,” said study senior author Gary Siuzdak, senior director of TSRI’s Scripps Center for Metabolomics and professor of chemistry, molecular and computational biology.

Using new capabilities within XCMS, a data analysis platform developed in the Siuzdak lab, the researchers observed small molecules transforming—and even disappearing—during an experiment meant to mimic the GC-MS process, throwing into question the nature of the data being generated by GC-MS.

The study was published online ahead of print on October 4 in the journal Analytical Chemistry.

A Closer Look at GC-MS

For more than 50 years, chemists and biologists have used GC-MS to identify and measure concentrations of small molecules. When a sample is injected in a GC-MS system, it is heated and vaporized. The vapor travels through a gas chromatography column and the molecules separate, allowing the mass spectrometer to measure the individual molecules in the sample. Today, GC-MS is widely used in thousands of laboratories for tasks such as chemical analysis, disease diagnosis, environmental monitoring and even forensic investigations.

The new experiments were initiated when Siuzdak was preparing a short course for students at the American Society for Mass Spectrometry annual meeting. The question arose of how heat from the GC-MS vaporization process could affect results, so Siuzdak and TSRI Research Associate Mingliang Fang ran a set of experiments to compare how small molecules responded to thermal stress.

To their surprise, the molecular profiles of as many as 40 percent of the molecules were altered, suggesting that heat from the GC-MS process could dramatically change the chemical composition of the samples.

“The results were quite astounding—as this is a technology that has been used for decades,” said Siuzdak.

The finding led the researchers to take a closer look at how molecules degrade and transform during GC-MS.

Heat Transforms Molecules

The scientists analyzed small molecule metabolites heated at 60, 100 and 250 degrees Celsius to mimic sample preparation and analysis conditions. The team used XCMS combined with a low-temperature liquid chromatography mass spectrometry technology which has been previously shown not to degrade molecules thermally, to determine the extent of the thermal effects.

The researchers observed significant degradation even at the lower temperatures. At the higher temperatures, almost half of the molecules were degraded or completely transformed.

“In retrospect, there was very little to be surprised about: heat degrades molecules,” said Siuzdak. “However we’ve simply taken for granted the extent of thermal degradation. While this is a negative result and scientists rarely publish them, I felt compelled especially for the students just getting started in their careers to report the limitation of such a ubiquitous technology.”

The researchers noted that even molecules not typically observed in GC-MS can also be transformed; for example, the energy metabolite adenosine triphosphate (ATP) was readily converted into adenosine monophosphate (AMP). This transformation is relevant for medical research because scientists often use a heating process to look at the ratio of ATP to AMP in cells to estimate the function of cellular components in aging and disease.

“People use this ratio to detect disease, but if the ratio can be changed by the heating process, the results will not be accurate,” said Fang. “It is known that ATP is thermally sensitive, but not how it changed under these conditions.”

Thermal degradation could also explain why many scientists have detected many unknown molecular “peaks” in the past. Based on the new study, the researchers now believe these metabolites may be byproducts of the heating process—the result of reactions between metabolites as they degrade.

The Next Step

So why hadn’t scientists figured out the effect of heating until now? Siuzdak explained that while some scientists had noticed changes in specific metabolites, it was difficult to see changes in overall molecular profiles that contain thousands of molecules.

This omic-based study was made possible by new capabilities within the XCMS program developed at the TSRI Scripps Center for Metabolomics. XCMS is a free, cloud-based data analysis technology used to analyze mass spectrometry data all over the globe.

“With XCMS, we could expand our study to obtain a global profile of how the metabolites were altered—not just a few compounds,” said Fang.

“Fortunately these problems can be overcome with the use of standards in GC-MS as well as using newer, ambient temperature mass spectrometry technologies, and this report will likely stimulate more scientists to move to these less destructive alternatives,” said Siuzdak.

In addition to Siuzdak and Fang, authors of the study, “Thermal Degradation of Small Molecules: A Global Metabolomic Investigation,” were Julijana Ivanisevic of the University of Lausanne, Michael E. Kurczy of Astrazeneca and TSRI, Gary J. Patti of Washington University in St. Louis; and Caroline H. Johnson, Linh T. Hoang, Winnie Uritboonthai and H. Paul Benton of TSRI. See http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b03003

This study was supported by the National Institutes of Health (grants R01 GM114368 and P01 A1043376-02S1)

Learn more about XCMS at xcmsonline.scripps.edu.

About The Scripps Research Institute

The Scripps Research Institute (TSRI) is one of the world’s largest independent, not-for-profit organizations focusing on research in the biomedical sciences. TSRI is internationally recognized for its contributions to science and health, including its role in laying the foundation for new treatments for cancer, rheumatoid arthritis, hemophilia, and other diseases. An institution that evolved from the Scripps Metabolic Clinic founded by philanthropist Ellen Browning Scripps in 1924, the institute now employs about 2,700 people on its campuses in La Jolla, CA, and Jupiter, FL, where its renowned scientists—including two Nobel laureates—work toward their next discoveries. The institute’s graduate program, which awards PhD degrees in biology and chemistry, ranks among the top ten of its kind in the nation. For more information, see www.scripps.edu.

 

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