The role of the placenta in healthy fetal development is under-appreciated according to a new paper. The paper available in the journal Nature, reveals a how 103 genetic mutations in mice cause embryos to die before birth. The results showed the majority, almost 70%, cause defects in the placenta.
Each of the 103 gene mutations caused a loss of a particular factor. Many of these had not been previously linked to placenta development, and the study highlights the unexpected number of genes affecting placenta development. By studying a select group of three genes in further detail, the team showed the death of the embryo could be directly linked to defects in the placenta in one out of these three cases. This may mean a significant number of genetic defects leading to prenatal death may be due to abnormalities of the placenta, not just the embryo.
“Our data highlight the hugely under-appreciated importance of placental defects in contributing to abnormal embryo development and suggest key molecular nodes governing placentation,” said Dr Myriam Hemberger, Group Leader in Epigenetics at Babraham Institute. “The importance of a healthy placenta has often been overlooked in these studies and it is important that we start doing more to understand its contribution to developmental abnormalities.”
Although the research uses mice, the findings are likely to be highly relevant to complications during human pregnancy. The study stresses the need for more work to be done on investigating development of the placenta during human pregnancies. The study was part of the Wellcome Trust-funded “Deciphering the Mechanisms of Developmental Disorders (DMDD)” consortium. Dr Myriam Hemberger at the Babraham Institute, Cambridge led the research, working with colleagues at the Wellcome Sanger Institute, Cambridge, the Francis Crick Institute, London, the University of Oxford and the Medical University of Vienna, Austria.
The placenta is vital for normal pregnancy progression and embryo development in most animals that give birth to live young, including humans. It provides a unique and highly specialised interface between the embryo and the mother, ensuring an adequate provision of nutrients and oxygen to the embryo. The placenta is also involved in waste disposal from the embryo and produces important hormones to help sustain pregnancy and promote fetal growth. Although previous research has highlighted the pivotal role of the placenta for a healthy pregnancy, its potential contribution to pregnancy complications and birth defects continues to be overlooked.
Scientists call mutations that cause death in the womb embryonic lethal. Mouse lethal genes are enriched for human disease genes and the affected embryos often show morphological abnormalities, i.e. changes to their shape and structure. Around one-third of all gene mutants studied in mouse are lethal or survivable (that is mutant offspring are less likely to survive than non-mutant pups).
The DMDD consortium investigates the detailed morphological abnormalities in the embryo caused by such gene mutations. Importantly, the DMDD programme includes analysis of placentas from these mutants. Through this uniquely comprehensive assessment, the team was able to show a significant co-occurrence of placental defects alongside abnormalities in the brain, heart and vascular system.
The placenta-focussed aspect of the DMDD project was carried out at the Babraham Institute where all mutant placentas were analysed and characterised for their morphological defects. The Institute researchers also carried out work using mouse placental stem cells to understand further the functional roles of particular genes in the development of this organ. The mutant mouse lines were produced and bred at the Wellcome Sanger Institute. The detailed morphological analysis of embryos was performed by Dr Tim Mohun at the Francis Crick Institute together with colleagues at the Division of Anatomy, Medical University of Vienna, Austria.