The study, “Deep Sequencing of Three Loci Implicated in Large-Scale Genome-Wide Association Study Smoking Meta-Analyses,” was published in August as an advanced online publication in the Oxford University Press journal Nicotine & Tobacco Research.
“We dug deeper into genes known to be associated with smoking,” said Shaunna L. Clark, Ph.D., research assistant professor, Center for Biomarker Research and Precision Medicine, VCU School of Pharmacy. Previous large-scale, genomewide association studies have identified three genes that are related to cigarette addiction, but the VCU-led study is the first to identify specific sets of genetic variants that might be responsible.
Researchers at the CBRPM sequenced the three genes and their adjacent regions to get a complete catalog of all the genetic variation that could be contributing to addiction. Sequencing the entire gene allowed Clark and her colleagues to examine variants that other studies had not addressed, such as rare variants not commonly found in the population and regulatory variants that can increase or decrease gene expression.
“We found that the tendency toward nicotine addiction is likely caused by many variants, each with a small effect,” Clark said. “Thus, multiple variants within the same gene are related to smoking.”
The study was supported by the National Institutes of Health (R01 DA024413, R01 MH045268, R01 MH068521, R25 DA026119, K01 AA021266 and K01 MH093731). Researchers from Duke University Medical Center and the University of North Carolina at Chapel Hill contributed to the study.
About VCU and VCU Health
Virginia Commonwealth University is a major, urban public research university with national and international rankings in sponsored research. Located in downtown Richmond, VCU enrolls more than 31,000 students in 226 degree and certificate programs in the arts, sciences and humanities. Sixty-seven of the programs are unique in Virginia, many of them crossing the disciplines of VCU’s 13 schools and one college. The only academic medical center and Level I trauma center in the region, VCU Health is comprised of five health sciences schools (Allied Health Professions, Dentistry, Medicine, Nursing, Pharmacy), VCU Medical Center, Community Memorial Hospital, Children’s Hospital of Richmond at VCU, VCU Massey Cancer Center and Virginia Premier. For more, please visit www.vcu.edu.